percentages of impaired tubules at 14 days after IRI were 73.2% in the control group and 55.6% in the fasting group.ATP recovery rates in the acute phase were inversely correlated with tubular damage in the chronic phase.Gene ontology enrichment analysis showed upregulation of fatty acid metabolic pathways in PT cells of fasted mice in comparison with the control group.In addition, the administration of a QM release probe successfully visualized the activation of -oxidation in the kidney at the single-cell level in vivo.Consequently, -oxidation was predominantly activated in PTs and was further upregulated under fasting conditions. Conclusion:The ATP recovery rate in PTs in the acute phase was improved in fasted mice, which was associated with reduced tubular injury in the chronic phase.Gene expression related to fatty acid metabolism was also elevated in PT cells of fasted mice.Moreover, QMreleasing probe administration demonstrated the activated -oxidation in PTs after fasting.These data suggest that enhanced -oxidation in the PT of fasting mice may be associated with improved ATP recovery rate and resistance to injury after IRI.This abstract was also submitted for JSN/ERA Symposium and ASN Kidney Week 2025 congress, and re-submission is permitted by JSN and ASN.
Goto et al. (Wed,) studied this question.