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Identify bioactive compounds in exosome-like nanoparticles from Sparassis crispa and evaluate their effects on collagen biosynthesis.

April 1, 2026

Chemical constituents of exosome‐like nanoparticles‐enriched vesicle fraction derived from Sparassis crispa and its biological and molecular docking studies

Key Points

Identify bioactive compounds in exosome-like nanoparticles from Sparassis crispa and evaluate their effects on collagen biosynthesis.
Isolation of three bioactive compounds from ELN-enriched vesicle fractions.
Assessment of COL1A1 gene expression in CCD-1064Sk human fibroblasts.
Molecular docking analysis with collagenase enzymes MMP-1, MMP-8, and MMP-13.
Sparalide C, 5-hydroxy-7-methoxyphthalide, and methyl orsellinate were identified as bioactive compounds.
Isolated compounds enhanced COL1A1 gene expression, promoting collagen biosynthesis.
Molecular docking indicated moderate binding affinities to collagenase enzymes, supporting their role in skin health.

Abstract

This study identified three bioactive compounds, sparalide C (1), 5-hydroxy-7-methoxyphthalide (2) and methyl orsellinate (3), from ELN-enriched vesicle fraction derived from S. crispa. All isolated compounds enhanced COL1A1 gene expression in CCD-1064Sk human fibroblasts, suggesting their potential to promote collagen biosynthesis. Molecular docking analysis further supported their relevance by indicating moderate binding affinities to collagenase enzymes (MMP-1, MMP-8 and MMP-13), which are known to degrade type I collagen. These findings suggest that ELN-enriched vesicle fraction derived from S. crispa may serve as a promising natural source for skin health applications, particularly in maintaining extracellular matrix homeostasis.

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Chemical constituents of exosome‐like nanoparticles‐enriched vesicle fraction derived from Sparassis crispa and its biological and molecular docking studies

Key Points

Abstract

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