Abstract 7147: CAR T-Cell therapy efficacy evaluation inTumorGraft3D tri-culture platform with reconstructed tumor microenvironment

Key Points

• This investigation aims to evaluate the efficacy of CAR T-cells in a complex tumor microenvironment.
• Developed the TumorGraft3D-Tri-Culture platform to mimic tumor interactions.
• Assessed CD70-directed CAR T-cells in renal cell carcinoma.
• Compared standard CAR T-cells with armored CAR T-cells resistant to TGFβ-mediated suppression.
• Used imaging and luminescence analyses to measure tumor viability and immune activation.
• Both CAR T-cell types reduced tumor viability compared to controls.
• Armored CAR T-cells displayed enhanced cytotoxicity and sustained activation.
• CD69 expression increased in both CAR T-cell types, indicating robust activation.
• CD70 surface expression on tumor cells decreased post-treatment, indicating effective target engagement.