Abstract 4157: Spatial immune profiling of solid tumors by complementing H&E with a novel 17-plex immunofluorescence approach
Key Points
To enhance the identification of cellular neighborhoods in solid tumors by integrating multiplex immunofluorescence with H&E staining.
Validated a 17-plex immunofluorescence assay on the Orion platform.
Aligned same-slide H&E images with fIHC to assess cellular neighborhoods.
Employed HALO software for cell phenotyping and slide registration.
Supported identification of regions with tumor-infiltrating lymphocytes and immune deserts.
Improved interpretability of spatial patterns by linking cell phenotypes with tissue morphology.
Demonstrated adaptability of the workflow across various tumor types.
Abstract
Abstract Background: Spatial biology has increasingly leveraged “spatial neighborhoods” to integrate how cells are organized and interact, yet standards for defining and identifying these neighborhoods are still developing. Multiplex fluorescence immunohistochemistry (fIHC) offers detailed profiling of immune and tumor cell states, while hematoxylin and eosin (H Part 1 (Regular Abstracts); 2026 Apr 17-22; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2026;86(7 Suppl):Abstract nr 4157.
Abstract 6193: Exploring interplay between tumor intrinsic features and immune cell phenotypes in multi-tumor tissue microarrays by a single step 15-plex fluorescence immunohistochemistry and imaging on the Orion platform2024 · 1 citations