August 30, 2004

Mechanism and use of the commercially available viability stain, BacLight

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Abstract

The mechanism is a combination of displacement of SYTO9 by PI and quenching of SYTO9 emissions by fluorescence resonance energy transfer. Confusing results can occur if the relative intensities of the stains or the concentration of PI relative to nucleic acid are not properly accounted for.

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Cite This Study

Stuart M. Stocks (Mon,) studied this question.

synapsesocial.com/papers/69dcbccf34612599f33593c5 https://doi.org/https://doi.org/10.1002/cyto.a.20069

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