Identification of EnzymeGene Families Related to Proline Biosynthesis and Metabolism in Pepper and Expression Analysis under Low Temperature and Low Light Stress

Key Points

• To identify enzyme gene families involved in proline biosynthesis and metabolism in pepper and analyze their expression under low temperature and low light stress.
• Conducted genome-wide identification of proline-related enzyme genes in Capsicum annuum.
• Analyzed gene expression under cold and low light stress conditions.
• Performed phylogenetic and collinearity analysis to assess evolutionary relationships.
• Investigated cis-acting elements associated with stress responses in the identified genes.
• Identified 10 enzyme genes related to proline biosynthesis and metabolism with varying isoelectric points.
• CaP5CS1 expression significantly upregulated under stress, while other proline degrading genes were downregulated.
• Phylogenetic analysis revealed close relationships with tomato and potato genes.