Insights into complementary exposomic targeted analysis and suspect screening approaches: a case study examining human serum for chemicals with LC-IMS-MS

Key Points

• The study aims to enhance the detection and quantitation of chemicals in human serum using integrated analytical methods.
• Developed a scalable workflow for chemical analysis in human serum.
• Integrated targeted quantitation of PFAS with LC-IMS-MS for suspect screening.
• Utilized collision cross section (CCS) for improved selectivity and annotation.
• Successfully improved selectivity in chemical detection within complex serum matrices.
• Demonstrated the effectiveness of combining targeted analysis with suspect screening.
• Achieved better annotation confidence for identified chemicals.