Simultaneous Quantitation of Structurally Analogous Bile Acid Isomers via Charge-State-Engineered UPLC-MS

Key Points

• The research aims to develop a method to simultaneously quantify bile acid isomers with similar molecular masses.
• Utilized charge-state-engineered ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) for analysis.
• Focused on the structural analogs of bile acids including ursodeoxycholic, hyodeoxycholic, chenodeoxycholic, and deoxycholic acid.
• Analyzed specific biological roles and complications of isomeric structures.
• Successfully identified and quantified the isomeric bile acids.
• Showed improved analytical separation compared to traditional methods.
• Highlighted significant challenges in separating structurally similar compounds.