Accuracy of estimated cell viability estimation during cold storage using electrical impedance measurement was investigated. Rat cardiac myoblasts (H9c2) were preserved at 4°C in ET-Kyoto solution or UW solition for 0 to 12 h. Cell viability was estimated using an impedance ratio (16 kHz / 100 kHz) and compared with actual cell viability evaluated by WST-8. As a result, up to 12 hours in ET-K and UW, the relative deviation remained within 10%. It was suggested that the cell viability estimated by using an electrical impedance ratio and standardization correction is united as satisfactorily as the actual cell viability.
Seno et al. (Wed,) studied this question.