Protocol for mapping murine myeloid bone marrow progenitors and their differentiation into CD103+ cDC1s and CD301b+ cDC2s.

Key Points

• To outline a protocol for mapping murine myeloid bone marrow progenitors and their differentiation.
• Use a specific mapping protocol to identify bone marrow progenitors
• Analyze differentiation pathways leading to CD103+ cDC1s and CD301b+ cDC2s
• Employ murine models for accurate results
• Identified key steps in the differentiation of myeloid progenitors
• Mapped the transition of progenitors to mature dendritic cell subsets
• Provided foundational insights into immune system development