What question did this study set out to answer?

The aim is to develop a fluorescent sensor for the detection of o-nitroaniline using a light-driven cascade.

May 7, 2026

An Analyte-Derived Turn-On Fluorescent Nanozyme Sensor Enabled by a Programmable Light-Driven Cascade for o-Nitroaniline Detection

Key Points

The aim is to develop a fluorescent sensor for the detection of o-nitroaniline using a light-driven cascade.
Utilized a programmed light-driven cascade to dynamically regulate electron flow.
Characterized the sensor for peroxidase-like activity.
Tested the sensor's detection range in real water matrices.
Achieved a linear detection range of 2.0-40 μM.
Obtained a detection limit of 0.23 μM.
Demonstrated reduced false positives from similar nitroaromatics.

Abstract

dynamically regulates electron flow and markedly enhances peroxidase-like activity to generate reactive oxygen species that efficiently oxidize the intermediate into the strongly emissive 2,3-diaminophenazine. This establishes a fundamentally new "self-luminescence" signal-from-none mechanism in which fluorescence arises uniquely from the target's directional conversion, thereby minimizing batch effects and suppressing false positives from structurally similar nitroaromatics. The platform exhibits a linear detection range of 2.0-40 μM and a detection limit of 0.23 μM, supporting compliance-oriented monitoring in real water matrices.

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