Methyl Proton Spin Relaxation ( R 2 / R 1 ) Enables Sensitive Detection of pH-Dependent Oligomerization in GLP-1 Analogs

Key Points

• The aim is to evaluate a method for detecting pH-dependent oligomerization of GLP-1 analogs using methyl proton spin relaxation.
• Used methyl proton spin relaxation (R2/R1) for analysis.
• Focus on pH-dependent interactions of peptide analogs.
• Applied the method for peptide stability control.
• Enhanced discriminatory power for detecting subtle oligomerization under varying pH conditions.
• Demonstrated practical application in peptide formulation development.
• Validated the effectiveness for ensuring peptide stability.