Identification of Novel Therapeutic Agent Candidates Through High Throughput Screening With Chemical Library Based on Molecular Subclassification in Canine Histiocytic Sarcoma Cell Lines

Key Points

• The aim is to identify new therapeutic agents for canine histiocytic sarcoma using high throughput screening of a chemical library.
• Investigated eight CHS cell lines classified into two groups based on molecular characteristics.
• Administered duvelisib to assess its effects on phosphorylated Akt levels, cell cycle arrest, and cell death.
• Duvelisib reduced phosphorylated Akt protein levels in Group A cell lines with a concentration of 7.46 μM.
• Induced cell cycle arrest and increased cell death in a subset of Group A cell lines.
• Minimal cytotoxicity was observed in normal cells.