Abstract Context: Solanesol is an active compound isolated from the leaves of Nicotiana tabacum. This compound has bioactive properties, including anti-inflammatory, antibacterial, antioxidant, and neuroprotective. This compound has the potential as a supporting ingredient of vital pulp therapy (VPT), including its ability to suppress the pro-inflammatory cytokine tumor necrosis factor-alpha (TNF-α). Aims: This study evaluates the potential combination of Solanesol with Ca(OH) 2 as an anti-inflammatory in human dental pulp stem cells (hDPSCs) by looking at TNF-α expression. Settings and Design: An in vitro experimental study conducted at ProSTEM (Prodia StemCell) Jakarta, Indonesia. Materials and Methods: Consists of six treatment groups, combinations of Solanesol ratios: Ca(OH) 2 1:1, 1:2, and 2:1, Solanesol, Ca(OH) 2 , and MTA. TNF-α levels were measured, and ELISA tests were performed on days 3, 7, 14, and 21. Statistical Analysis Used: Data were analyzed using the one-way ANOVA followed by Tukey’s post hoc test ( P < 0.05). Results: Statistically, the combination of Solanesol and Ca(OH) 2 ratios did not demonstrate a significant difference in reducing TNF-α levels compared to the Ca(OH) 2 and MTA groups. However, the Solanesol-only group exhibited a decrease in TNF-α levels. Conclusions: The combination of Solanesol and Ca(OH) 2 was shown to suppress the expression of the pro-inflammatory cytokine TNF-α in hDPSCs. Furthermore, Solanesol shows potential as a biomaterial with anti-inflammatory properties and may be developed as a bioactive component for use in VPT applications.
Widyastuti et al. (Wed,) studied this question.