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Purification of a bone-inducing substance (osteogenic factor) from a murine osteosarcoma is reported. The final preparation of the osteogenic factor appeared homogeneous in Sephacryl S-200 gel chromatography and sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis. The factor is a glycoprotein with a molecular weight of 22,000. The yield of the factor was 1 mg from l0 g of osteosarcoma. Allo-implantation of the factor provoked ectopic bone formation in situ in three to four weeks.
Takaoka et al. (Thu,) studied this question.