What question did this study set out to answer?

The aim is to see if nonylphenol exposure in dams during the perinatal period leads to airway inflammation in pups and affects OVA-induced asthma.

May 20, 2026

C70-18 Effects of Nonylphenol Exposed in Dams During Perinatal Period on Airway Inflammation in Ova-Induced Asthmatic Pup

Key Points

The aim is to see if nonylphenol exposure in dams during the perinatal period leads to airway inflammation in pups and affects OVA-induced asthma.
Dams divided into 5 groups with varying nonylphenol doses and estradiol as control.
Gavage administered daily from day 7 of pregnancy to 21 days after birth.
Assessments of respiratory and inflammatory changes in pups post-exposure.
In the 100 mg/kg NP group, significant increases in HMGB1, NF-κB expression, and inflammatory cell infiltration were observed.
In the OVA group, IL-4 levels increased while IFN-γ levels decreased.
100 mg/kg NP+OVA group showed greater inflammation and structural lung changes compared to controls.

Abstract

Abstract Objective To investigate whether exposure to NP in dams during perinatal period causes airway inflammation in pups, and whether NP affects airway inflammation in OVA-induced asthmatic pups. Methods Dams were randomly divided into 5 groups (negative control group, 25 mg/kg NP group, 50 mg/kg NP group, 100 mg/kg NP group and 30 μg/kg E2 group), with the everyday gavage of the volume 5 ml/kg/d from the 7th day of pregnancy to 21st days after the birth of the pups. Results In the 100 mg/kg NP group: the respiratory rhythm was slightly faster; the lung tissue was slightly looser; the bronchial smooth muscle layer was slightly thickened with inflammatory cell infiltration; the osmiophilic multilamellar body of the type II epithelial cells was partially emptied; and lymphocytes and neutrophils were the main cells in Balf; the relative expression of HMGB1, NF-κB genes and proteins in lung tissue of 100 mg/kg NP group was significantly higher than that in the control group. The expression level of TGF-β1 mRNA in the lung tissue of 100 mg/kg NP group was lower than that in the 30 μg/kg E2 group. NP concentration in the lung tissue of pups was positively correlated with expression levels of HMGB1, NF-κB genes and protein. 2) In the OVA group: the levels of IL-4 in Balf supernatant and OVA-sIgE in serum were significantly higher, while the level of IFN-γ in Balf supernatant was significantly lower. 3) In the 100 mg/kg NP+OVA group: the rubbing frequency of the head and upper body in the glass atomizing box significantly increased; the lung tissue was significantly looser; the NP concentration was significantly higher; the bronchial smooth muscle layer was significantly thickened and the inflammatory cell exudation significantly increased in the lumen; the mitochondria in type II epithelial cells was significantly swelling; the relative expression of HMGB1, NF-κB genes and proteins in lung tissue showed an increasing trend. The expression level of ER-βproteins in the lung tissue of 30 μg/kg E2+OVA group was significantly higher than that in 100 mg/kg NP+OVA group. NP concentration in the lung tissue of asthmatic pups was positively correlated with HMGB1 protein expression. Conclusion 1) Dams exposed to 100 mg/kg NP might aggravate airway inflammation in pups. 2) Dams exposed to 25 mg/kg NP can reduce airway inflammation in OVA-induced asthmatic pups; however, dams exposed to 100 mg/kg NP aggravate the degree of airway inflammation. This abstract is funded by: National Natural Science Foundation of China (22266039, 22166035)

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