Simultaneous PET-MR imaging with targeted probes detected elevated myocardial MRI percent signal increase in TAC mice compared to sham (29±15 vs 6.1±4, P<0.0001).
A dual molecular PET-MR imaging protocol successfully detected cardiopulmonary fibrosis and fibrogenesis in a mouse model of left ventricular dysfunction.
Absolute Event Rate: 29% vs 6.1%
p-value: p=<0.0001
Abstract Background Aging-associated left ventricular (LV) dysfunction promotes cardiopulmonary fibrogenic remodeling, Group 2 pulmonary hypertension (PH), and right ventricular failure. At the time of diagnosis, cardiac function has declined, and cardiopulmonary fibrosis has often developed. Here, we sought to develop a molecular positron emission tomography (PET)-magnetic resonance imaging (MRI) protocol to detect both cardiopulmonary fibrosis and fibrotic disease activity in an LV dysfunction model. Methods LV dysfunction was induced by transverse aortic constriction (TAC) in 6-month-old senescence-accelerated prone (SAMP8) mice, a subset of mice received sham surgery. Three weeks post-surgery, mice underwent simultaneous PET-MR imaging at 4.7 T. Collagen–targeted PET and fibrogenesis MR probes were intravenously administered. PET signal was computed as myocardium- or lung-to-muscle ratio (MMR, LMR). Percent signal increase (%SI) and ΔLMR were computed from the pre-/post-injection MR images. Tissue specimens were analyzed for hydroxyproline (Hyp) and allysine content. Ventricular structure and function were measured by echocardiography and hemodynamic pressure-volume (PV) loop analysis. Results Allysine in the heart (22±5 (TAC), 13±5 (sham) nmol/g, P=0.02 ) and lungs (7.5±4 (TAC), 5.8±2 (sham) nmol/lung, P=0.17 ) of TAC mice corresponded to an increase in myocardial MRI %SI (29±15 (TAC), 6.1±4 (sham), P<0.0001 ) and ΔLMR (0.3±0.1 (TAC), 0.08±0.1 (sham), P<0.0001 ). Hyp in the heart (555±90 (TAC), 400±80 (sham) µg/g, P<0.0001 ) and lungs (189±63 (TAC), 143±31 (sham) µg/lung, P<0.01 ) were elevated in TAC mice, which corresponded to an increase in PET signal (MMR: 1.8±0.1 (TAC), 1.6±0.2 (sham), P=0.02 ; LMR: 1.5±0.1 (TAC), 1.2±0.1 (sham), P<0.001 ). PV loop and echocardiography demonstrated adverse LV remodeling, function, and increased right ventricular systolic pressure in TAC mice. Conclusions Administration of collagen-targeted PET and allysine-targeted MR probes led to elevated PET-MRI signals in the myocardium and lungs of TAC mice. The study demonstrates the potential to detect fibrosis and fibrogenesis in cardiopulmonary disease through a dual molecular PET-MR imaging protocol.
Moon et al. (Sun,) conducted a other in Left ventricular dysfunction and cardiopulmonary fibrosis. Transverse aortic constriction (TAC) and PET-MR imaging vs. Sham surgery was evaluated on Myocardial MRI percent signal increase (%SI) (p=<0.0001). Simultaneous PET-MR imaging with targeted probes detected elevated myocardial MRI percent signal increase in TAC mice compared to sham (29±15 vs 6.1±4, P<0.0001).