May 1, 1987Open Access
Effect of internal viral sequences on the utility of retroviral vectors
Structured PICO
P
Population
Moloney murine leukemia virus-based vector expressing human ADA cDNA
I
Intervention
Inclusion of intron-contained sequences and viral gag gene sequences
O
Outcome
Virus titer generation and cDNA expressionsurrogate
Inclusion of viral gag gene sequences in Moloney murine leukemia virus-based vectors significantly increases viral titers, improving retroviral vector utility.
Abstract
Expression of the human ADA cDNA encoded by the Moloney murine leukemia virus spliced RNA form is enhanced by intron-contained sequences. The presence of sequences corresponding to the viral gag gene in a Moloney murine leukemia virus-based vector results in the generation of 10- to 40-fold higher titers of virus.