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August 19, 1983Science141 citations

Multigene Family for Sarcomeric Myosin Heavy Chain in Mouse and Human DNA: Localization on a Single Chromosome

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LLLeslie A. LeinwandRFR. E. K. FournierBNBernardo Nadal‐Ginard

Key Points

  • This research aims to map the localization of sarcomeric myosin heavy chain genes in mouse and human genomes.
  • Cloned myosin heavy chain DNA probes from rat and human were hybridized with genomic DNA.
  • Restriction endonuclease digests of somatic cell hybrids and parental cells were used.
  • The localization of myosin heavy chain genes was determined on mouse chromosome 11 and human chromosome 17.
  • Mouse myosin heavy chain genes were located on chromosome 11.
  • Three different human myosin heavy chain genes were mapped to chromosome 17.
  • Synteny was preserved between myosin heavy chain and markers thymidine kinase and galactokinase in both genomes.

Abstract

Cloned myosin heavy chain DNA probes from rat and human were hybridized to restriction endonuclease digests of genomic DNA from somatic cell hybrids and their parental cells. The mouse myosin heavy chain genes detectable by this assay were located on chromosome 11, and three different human sarcomeric myosin heavy chain genes were mapped to the short arm of chromosome 17. A synteny between myosin heavy chain and two unrelated markers, thymidine kinase and galactokinase, was found to be preserved in the rodent and human genomes.

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Cite This Study

Leinwand et al. (1983) studied this question.

synapsesocial.com/papers/6a10daf2acd1dbe0646483adhttps://doi.org/10.1126/science.6879174
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