ABSTRACT To address inadequate interlayer integration and insufficient vascularization in guided tissue regeneration (GTR) membranes, this study employs a unidirectional nanopore dehydration technology previously developed by our group to fabricate a polyvinyl alcohol/egg white (PVA/EW) Janus hybrid membrane. Scanning electron microscopy revealed seamless integration between dense (pore diameter: 0.2−2 µm) and porous layers (pore diameter: 10−100 µm) without distinct interfaces. Crucially, EW was enriched in the porous layer (validated by Fourier transform infrared spectroscopy), endowing the membrane with enhanced bioactivity. After tannic acid‐assisted biomimetic mineralization, the PVA/EW20‐H membrane achieved a tensile stress of 2.38 ± 1.09 MPa and strain of ∼650%, representing about a 4‐fold increase in strength compared to non‐mineralized counterparts. The membrane exhibited selective permeability, allowing approximately 40% cumulative release of 4 kDa FITC‐dextran over 50 h while effectively blocking L929 fibroblast infiltration. In vitro, the membranes promoted HUVEC migration, suggesting a potential to support early angiogenic processes, and enhanced osteogenesis in MC3T3‐E1 cells, as assessed by alkaline phosphatase/alizarin red S staining and qRT‐PCR. Thus, our work represents a facile, green, and biocompatible PVA/EW membrane, offering a promising candidate for periodontal regeneration that warrants further investigation.
Guo et al. (Fri,) studied this question.