Post-myocardial infarction remodelling in murine cardiomyocytes involves increased myofibrillar protein carbonylation and decreased Ca(2+) sensitivity of force production (pCa50 5.81 vs 5.91).
Increased myofibrillar protein carbonylation contributes to decreased Ca(2+) sensitivity and contractile dysfunction in the infarcted LV region during post-MI remodeling.
Absolute Event Rate: 5.81% vs 5.91%
AIMS: The region-specific mechanical function of left ventricular (LV) murine cardiomyocytes and the role of phosphorylation and oxidative modifications of myofilament proteins were investigated in the process of post-myocardial infarction (MI) remodelling 10 weeks after ligation of the left anterior descending (LAD) coronary artery. METHODS AND RESULTS: Permeabilized murine cardiomyocytes from the remaining anterior and a remote non-infarcted inferior LV area were compared with those of non-infarcted age-matched controls. Myofilament phosphorylation, sulfhydryl (SH) oxidation, and carbonylation were also assayed. Ca(2+) sensitivity of force production was significantly lower in the anterior wall (pCa50: 5.81 ± 0.03, means ± SEM, at 2.3 µm sarcomere length) than that in the controls (pCa50: 5.91 ± 0.02) or in the MI inferior area (pCa50: 5.88 ± 0.02). The level of troponin I phosphorylation was lower and that of myofilament protein SH oxidation was higher in the anterior location relative to controls, but these changes did not explain the differences in Ca(2+) sensitivities. On the other hand, significantly higher carbonylation levels, e.g. in myosin heavy chain (MHC) and actin were observed in the MI anterior wall carbonylation index (CI), CIMHC: 2.06 ± 0.46, CIactin: 1.46 ± 0.18 than in the controls (CI: 1). In vitro Fenton-based myofilament carbonylation in the control cardiomyocytes also decreased the Ca(2+) sensitivity of force production irrespective of the phosphorylation status of the myofilaments. Furthermore, the Ca(2+) sensitivity correlated strongly with myofilament carbonylation levels in all investigated samples. CONCLUSION: Post-MI myocardial remodelling involves increased myofibrillar protein carbonylation and decreased Ca(2+) sensitivity of force production, leading potentially to contractile dysfunction in the remaining cardiomyocytes of the infarcted area.
Balogh et al. (2013) studied Post-myocardial infarction remodelling. Ligation of the left anterior descending (LAD) coronary artery vs. Non-infarcted age-matched controls was evaluated on Ca(2+) sensitivity of force production (pCa50). Post-myocardial infarction remodelling in murine cardiomyocytes involves increased myofibrillar protein carbonylation and decreased Ca(2+) sensitivity of force production (pCa50 5.81 vs 5.91).