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An electrochemical method based on differential pulse voltammetry (DPV) employing a glassy carbon electrode was developed for the quantification of H 2 O 2 in Manihot esculenta Crantz cell suspensions derived from tuber explants. Cell cultures were exposed to different concentrations of cadmium (Cd 2+ ) for 24, 48, and 72 h. The analytical protocol exhibited a limit of detection of 10.28 μmol l −1 and a limit of quantification of 51.81 μmol l −1 . Cadmium treatment induced a transient increase in H 2 O 2 levels, consistent with an oxidative burst followed by antioxidant-mediated regulation. This behaviour reflects the dynamic activation of redox signalling pathways in cassava cells under metal stress. Overall, the findings indicate that M. esculenta Crantz may serve as a promising sedentary biomarker for cadmium contamination, providing a sensitive, reproducible, and cost-effective strategy for environmental monitoring in tropical regions.
Fonseca et al. (Fri,) studied this question.