What does this research mean for the field?

Glycerol-based deep eutectic solvents enhance the thermal stability of laccase, while the specific preparation history of these solvents uniquely modulates the enzyme's activation and overall behavior. Novelty: ClaimNovelty.INCREMENTAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

What question did this study set out to answer?

This research aims to investigate how preparation methods influence the activation and stabilization of laccase enzymes in glycerol-based deep eutectic solvents.

May 31, 2026Open Access

Preparation-dependent activation and stabilization of laccase in glycerol-based deep eutectic solvents

Key Points

This research aims to investigate how preparation methods influence the activation and stabilization of laccase enzymes in glycerol-based deep eutectic solvents.
Evaluated thermal stability of laccase in various glycerol-based deep eutectic solvents.
Compared aqueous mixtures of deep eutectic solvent components.
Analyzed the effects of preparation history on enzymatic behavior.
Glycerol-based deep eutectic solvents markedly enhanced the thermal stability of laccase.
Simple mixtures of deep eutectic solvent components improved enzyme stability.
Unique activation effects observed with glycerol–proline DES-derived solutions.

Abstract

• Glycerol-based DESs markedly enhanced the thermal stability of laccase. • Simple aqueous mixtures of DES components also improved enzyme stability. • Glycerol–proline DES-derived solutions showed unique activation effects. • Preparation history altered enzymatic behavior in glycerol-based DES systems. • Preparation-induced side reactions likely modulated laccase activity.

Preparation-dependent activation and stabilization of laccase in glycerol-based deep eutectic solvents

Key Points

Abstract

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