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October 1, 2003Journal of Biological ChemistryOpen Access

Differential Contribution of Inhibitory Phosphorylation of CDC2 and CDK2 for Unperturbed Cell Cycle Control and DNA Integrity Checkpoints

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Authors

JCJeremy P.H. ChowWSWai Yi SiuHHHorace T. B. Ho

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Overview

Randomized trial evaluates the role of inhibitory phosphorylation in cell cycle checkpoints, indicating distinct functions for CDC2 and CDK2.

Key Points

  • This research aims to evaluate how inhibitory phosphorylation of CDC2 and CDK2 contributes to cell cycle control and DNA integrity checkpoints.
  • Compared the roles of Thr14/Tyr15 phosphorylation in cyclin A/B1-CDC2 and cyclin A/E-CDK2 complexes in HeLa cells.
  • Analyzed effects of nonphosphorylation mutants on cell cycle progression and response to DNA damage.
  • Investigated downstream phosphorylation events relevant to S phase and mitotic control after DNA damage.
  • Inhibitory phosphorylation significantly regulates CDC2 but has a minor role for CDK2 in normal cell cycle progression.
  • Nonphosphorylation mutants of CDC2 and CDK2 induced histone H3 phosphorylation and cytotoxicity, even after DNA damage.
  • Specific regulators such as CDC25A and CDC25B altered histone H3 phosphorylation dynamics post-DNA damage.

Cite This Study

Chow et al. (2003) studied this question.

synapsesocial.com/papers/6a1c201a0a1f7575939d8da1https://doi.org/10.1074/jbc.m306683200
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