The establishment of a complete molecular map of brain aging is an important means for the study of epigenetics and transcriptomics in the aging process. Most of the research fields are focused on non-human primates. A variety of methods are used to analyze the regulatory mechanism of aging from different levels, reveal new gene regulatory networks of nervous system aging, and find new targets for anti-aging. These provide new theoretical basis and targets for delaying brain aging. This study is based on snRNA-seq, snATAC-seq and Stereo-seq studies. After sample screening, quality control and grouping, Eight different brain regions of cynomolgus monkeys at four different ages (young, middle, old and very old) were sequenced and library built by bioinformatics, and the molecular biological map of brain aging in macaca fascicularis was drawn by analysis. In addition, nuclear magnetic resonance imaging (MRI), immunohistochemistry (IHC) and immunofluorescence (IF) were used to verify the aging mechanism. To identify the key potential regulatory factors and gene components related to brain aging as targets, and to validate them downstream. This study aims to provide a basic theory for health protection in the process of human aging, provide a mechanistic research route for the diagnosis and treatment of brain diseases, screen out targeted genes that can regulate behavioral degradation in the process of aging or disease, and open up a potential new therapeutic approach for aging-induced neurodegenerative diseases. So as to promote the research in the field of life science and medicine.
赖光耀(Guangyao Lai) (Thu,) studied this question.