Synthesis and characterization of a novel phosphatidylinositol 5-phosphate (PI(5)P) photoaffinity probe

Key Points

• The aim is to develop and characterize a new photoaffinity probe for PI(5)P binding proteins.
• Synthesis of a novel PI(5)P photoaffinity probe.
• Characterization involved copper-catalyzed alkyne-azide click reactions.
• Used known PI(5)P binding proteins hUHRF1 and hTAF3 with dyes and affinity tags.
• Successfully synthesized and characterized the PI(5)P photoaffinity probe.
• Demonstrated interactions with binding proteins hUHRF1 and hTAF3 through labeled reactions.