Patients with heart failure who died or were rehospitalised had 51 significantly up-regulated and 46 down-regulated proteins compared to matched controls, highlighting altered metabolic pathways.
Case-Control (n=100)
Does the plasma proteome differ between heart failure patients who experience death or rehospitalisation compared to those who do not?
Plasma proteomic analysis reveals that glutathione, arginine, proline, and pyruvate metabolic pathways are activated in heart failure patients who experience death or rehospitalisation, suggesting potential novel therapeutic targets.
AIMS: To provide insights into pathogenesis of disease progression and potential novel treatment targets for patients with heart failure by investigation of the plasma proteome using network analysis. METHODS AND RESULTS: The plasma proteome of 50 patients with heart failure who died or were rehospitalised were compared with 50 patients with heart failure, matched for age and sex, who did not have an event. Peptides were analysed on two-dimensional liquid chromatography coupled to tandem mass spectrometry (2D LC ESI-MS/MS) in high definition mode (HDMSE). We identified and quantified 3001 proteins, of which 51 were significantly up-regulated and 46 down-regulated with more than two-fold expression changes in those who experienced death or rehospitalisation. Gene ontology enrichment analysis and protein-protein interaction networks of significant differentially expressed proteins discovered the central role of metabolic processes in clinical outcomes of patients with heart failure. The findings revealed that a cluster of proteins related to glutathione metabolism, arginine and proline metabolism, and pyruvate metabolism in the pathogenesis of poor outcome in patients with heart failure who died or were rehospitalised. CONCLUSIONS: Our findings show that in patients with heart failure who died or were rehospitalised, the glutathione, arginine and proline, and pyruvate pathways were activated. These pathways might be potential targets for therapies to improve poor outcomes in patients with heart failure.
Cao et al. (Wed,) conducted a case-control in Heart failure (n=100). Death or rehospitalisation vs. No event was evaluated on Differentially expressed proteins. Patients with heart failure who died or were rehospitalised had 51 significantly up-regulated and 46 down-regulated proteins compared to matched controls, highlighting altered metabolic pathways.