Combined nitric oxide and prostaglandin inhibition during hypoxic exercise blunts augmented vasodilation and hyperemia by approximately 50% compared with control conditions.
What are the roles of nitric oxide and prostaglandins in regulating skeletal muscle vasodilation during systemic hypoxia in humans?
Nitric oxide and prostaglandins are obligatory for hypoxic vasodilation in resting human skeletal muscle, but other local substances contribute during hypoxic exercise.
In humans, the net effect of acute systemic hypoxia in quiescent skeletal muscle is vasodilation despite significant reflex increases in muscle sympathetic vasoconstrictor nerve activity. This vasodilation increases tissue perfusion and oxygen delivery to maintain tissue oxygen consumption. Although several mechanisms may be involved, we recently tested the roles of two endothelial-derived substances during conditions of sympathoadrenal blockade to isolate local vascular control mechanisms: nitric oxide (NO) and prostaglandins (PGs). Our findings indicate that 1) NO normally plays a role in regulating vascular tone during hypoxia independent of the PG pathway; 2) PGs do not normally contribute to vascular tone during hypoxia, however, they do affect vascular tone when NO is inhibited; 3) NO and PGs are not independently obligatory to observe hypoxic vasodilation when assessed as a response from rest to steady-state hypoxia; and 4) combined NO and PG inhibition abolishes hypoxic vasodilation in human skeletal muscle. When the stimulus is exacerbated via combined submaximal rhythmic exercise and systemic hypoxia to cause further red blood cell (RBC) deoxygenation, skeletal muscle blood flow is augmented compared with normoxic exercise via local dilator mechanisms to maintain oxygen delivery to active tissue. Data obtained in a follow-up study indicate that combined NO and PG inhibition during hypoxic exercise blunts augmented vasodilation and hyperemia compared with control (normoxic) conditions by ∼50%; however, in contrast to hypoxia alone, the response is not abolished, suggesting that other local substances are involved. Factors associated with greater RBC deoxygenation such as ATP release, or nitrite reduction to NO, or both likely play a role in regulating this response.
Frank A. Dinenno (2015) conducted a review in Systemic hypoxia. Combined NO and PG inhibition vs. Control (normoxic) conditions was evaluated on Skeletal muscle vasodilation and hyperemia. Combined nitric oxide and prostaglandin inhibition during hypoxic exercise blunts augmented vasodilation and hyperemia by approximately 50% compared with control conditions.