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July 1, 1985The Journal of PhysiologyOpen Access

Influence of a change in stimulation rate on action potentials, currents and contractions in rat ventricular cells.

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Population

Ventricular cells isolated from rat heart

Comparison

Increase in stimulation rate from 0.3 to 3 Hz… vs Baseline stimulation rate of 0.3 Hz

Design

Preclinical

Key result

Increasing the stimulation rate from 0.3 to 3 Hz in isolated rat ventricular cells caused a gradual reduction in contraction amplitude and attenuation of the late phase of the action potential.

Authors

MMMary R. MitchellTPTheresa L. PowellDTDerek A. Terrar

Discussion

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Overview

No immediate clinical implications from rat cells; leaves open translation of rate-dependent calcium effects to human arrhythmias.

Structured PICO

P
Population
Ventricular cells isolated from rat heart
I
Intervention
Increase in stimulation rate from 0.3 to 3 Hz (or 4.2 Hz) using brief depolarization pulses or step depolarizations (10 or 100 ms)
C
Comparator
Baseline stimulation rate of 0.3 Hz
O
Outcome
Amplitude of contraction, action potential phase, second inward current (Isi), and extra contractions/currentssurrogate

Increasing stimulation rate in rat ventricular cells alters action potential duration, inward currents, and contraction amplitude, effects that are dependent on depolarization duration and intracellular calcium handling.

Cite This Study

Mitchell et al. (1985) studied this question. Increase in stimulation rate vs. 0.3 Hz was evaluated on Amplitude of contraction and late phase of the action potential. Increasing the stimulation rate from 0.3 to 3 Hz in isolated rat ventricular cells caused a gradual reduction in contraction amplitude and attenuation of the late phase of the action potential.

synapsesocial.com/papers/6a3f8039f157b8c38b07c3b2https://doi.org/10.1113/jphysiol.1985.sp015734
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