Administration of α-galactosylceramide significantly improved 28-day survival compared to PBS (59% vs 32%, P<0.05) and attenuated left ventricular remodeling in mice after myocardial infarction.
Does activation of iNKT cells with α-galactosylceramide improve survival and attenuate LV remodeling in mice after myocardial infarction?
Activation of iNKT cells with α-galactosylceramide improves survival and attenuates post-infarct LV remodeling in mice, mediated by enhanced IL-10 expression.
Absolute Event Rate: 59% vs 32%
p-value: p=<0.05
RATIONALE: Chronic inflammation in the myocardium is involved in the development of left ventricular (LV) remodeling and failure after myocardial infarction (MI). Invariant natural killer T (iNKT) cells have been shown to produce inflammatory cytokines and orchestrate tissue inflammation. However, no previous studies have determined the pathophysiological role of iNKT cells in post-MI LV remodeling. OBJECTIVE: The purpose of this study was to examine whether the activation of iNKT cells might affect the development of LV remodeling and failure. METHODS AND RESULTS: After creation of MI, mice received the injection of either α-galactosylceramide (αGC; n=27), the activator of iNKT cells, or phosphate-buffered saline (n=31) 1 and 4 days after surgery, and were followed during 28 days. Survival rate was significantly higher in MI+αGC than MI+PBS (59% versus 32%, P<0.05). LV cavity dilatation and dysfunction were significantly attenuated in MI+αGC, despite comparable infarct size, accompanied by a decrease in myocyte hypertrophy, interstitial fibrosis, and apoptosis. The infiltration of iNKT cells were increased during early phase in noninfarcted LV from MI and αGC further enhanced them. It also enhanced LV interleukin (IL)-10 gene expression at 7 days, which persisted until 28 days. AntienIL-10 receptor antibody abrogated these protective effects of αGC on MI remodeling. The administration of αGC into iNKT cell-deficient Jα18(-/-) mice had no such effects, suggesting that αGC was a specific activator of iNKT cells. CONCLUSIONS: iNKT cells play a protective role against post-MI LV remodeling and failure through the enhanced expression of cardioprotective cytokines such as IL-10.
Sobirin et al. (Sun,) conducted a other in Myocardial infarction (n=58). α-galactosylceramide (αGC) vs. Phosphate-buffered saline was evaluated on Survival rate (p=<0.05). Administration of α-galactosylceramide significantly improved 28-day survival compared to PBS (59% vs 32%, P<0.05) and attenuated left ventricular remodeling in mice after myocardial infarction.