Coexistence of two types of Ca(2+)-activated K+ channels in rat renal arterioles

Single-channel K+ currents were characterized in vascular smooth muscle cells freshly isolated from preglomerular arterioles (15-40 microns OD) of the rat. Under conditions of symmetrical K+ (145 mM), two types of single-channel K+ currents with unitary slope conductances of 68 +/- 2.6 and 251 +/- 4.9 pS were recorded from excised inside-out membrane patches. The open state probability (NPo) of these two types of K+ channels was voltage sensitive and the channels were highly selective for K+ over Na+. Elevation of intracellular calcium concentration (Ca2+i) from 0.1 to 0.5 microM on the cytoplasmic face of inside-out patches increased the frequency of opening and the NPo of both the 68-pS and the 251-pS K+ channels. Application of ATP (0.1-1 mM) to the internal surface of inside-out patches had no effect on the activities of both channel types. Internally applied Ba2+ (1 mM) blocked both of these channels. Externally applied tetraethylammonium (0.1-0.3 mM) or charybdotoxin (50 nM) blocked both the 68-pS and the 251-pS K+ channels. Externally applied apamin (50 nM), however, selectively blocked the 68-pS K+ channel but had no effect on the frequency of opening of the 251-pS K+ channel. Apamin also reduced macroscopic K+ current recorded from voltage-clamped rat renal arteriolar muscle cells by 25-30%. These results indicate the coexistence of two types of Ca(2+)-activated K+ channels in the membranes of vascular smooth muscle cell isolated from renal preglomerular arterioles of the rat that differ in unitary conductances and pharmacological properties.