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The L-2 light chain (DTNB light chain) was separated from rabbit skeletal muscle myosin and the amino acid sequence determined. In order to study the primary structure of the L-2 lihe amino acid seqence determined. Then, to determine the arrangement of these tryptic peptides, enzymatic partial hydrolysis using 0.25 M acetic acid were carried out. The primary structure of the L-2 light chain thus obtained contains 168 amino acids.
Matsuda et al. (1977) studied this question.