Supplementary Figure S4 from Multiomic mapping of acquired chromosome 1 copy number and structural variants to identify therapeutic vulnerabilities in multiple myeloma.

Chromatin state of varying deleted regions. There is a greater variability in terms of chromatin composition of the lost regions in comparision to the region of gain. The D1 region had identical an A-compartment percentage (56%) across all samples and reflected loss of significant subtelomeric region, including TNFRSF4. The D2 region was consistent across all samples (range 96-100% A-compartment) with the exception of KMS11 (47%), however this switch to a B-compartment occurred in a gene-sparse region. The D3 region encompasses the tumour suppressor genes CDKN2C and FAF1 and had similar active chromatin profiles (range 44-77%) except in RPMI8226 (0%). The D4 and D5 regions lie completely (100%) and near completely (>87%) in inactive compartments. Interestingly, the small A-compartments in D5 across U266 and RPMI8226 correspond to the tumour suppressor FUBP1. The D6 region showed conserved A-compartment across the samples specifically at the RPL5 and MTF2 loci while EVI5 and GFI1 showed switching from B-A across the differentiation stages. The D7 region, focused on TENT5C lies within an A-compartment across all samples except for KMS11, NBC=Naïve B-cell, MBC=Memory B-cell, GCBC=Germinal Center B-cell, PC=plasma cell.