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The rapid development of the PCR method opens up new opportunities for science and diagnostics. At the same time, the steady increase in the number of studies conducted increases the risk of contamination and, as a consequence, false-positive results. Today there are many ways to solve this problem, but none of them is universal. This paper presents the results of a comparison of the use of disinfectants and special decontamination agents to eliminate contamination of laboratory surfaces with nucleic acid amplification products – PCR amplicons. Study of decontamination activity against short DNA fragments of 117 bp were carried out under conditions that most closely simulate contamination of laboratory surfaces with PCR products. The effectiveness of decontamination was assessed by the residual number of amplicons in swabs from working surfaces. The high efficiency of destruction of amplicons when treating plastic, glass and metal surfaces with special decontamination agents based on metal ions and disinfectants based on oxygen-containing compounds has been shown. At the same time, it has been shown that agents containing a peroxide radical are capable of destroying amplicons mainly in combination with UV irradiation. Based on the results obtained, the feasibility of using metal ion-based decontamination agents for rapid and effective surface treatment in laboratories using nucleic acid amplification methods was demonstrated. Keywords: disinfectants, PCR, amplicons, decontamination.
Zamotaeva et al. (Sun,) studied this question.