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: It was found that there was no official method for simultaneous determination of luliconazole and beclomethasone dipropionate. : A new, straightforward, reliable, and highly precise HPLC technique for regular quality control analysis of the combination of Luliconazole and Beclomethasone dipropionate in a topical dosage form. : For the purpose of separation, 0.1% Glacial acetic acid: Acetonitrile (30:70) was utilised as the mobile phase. Column used was C18 (4.6 X150mm, 5µm) with flow rate of 1.0ml/min. Methanol was used as solvent. : Detection wavelength was 290nm and 242nm, measured at an Rt of 6.6 and 10.2 min. 13 minutes running time, Linearity and range were observed at concentrations from 5µg/ml to 150µg/ml and 0.4µg/ml to 4.8µg/ml of luliconazole and beclomethasone dipropionate respectively. The method developed was linear with a correlation coefficient of 0.999. : Validation of the method was performed according to ICH guidelines for assay, linearity and range, precision, limit of detection, limit of quantitation, and forced degradation test.
Kolhe et al. (Thu,) studied this question.