Key points are not available for this paper at this time.
NiFe hydrogenases catalyze the reversible cleavage of molecular hydrogen into protons and electrons. Here, we have studied the impact of temperature and illumination on an oxygen-tolerant and thermostable NiFe hydrogenase by IR and EPR spectroscopy. Equilibrium mixtures of two catalytic NiFe states, Ni
Karafoulidi‐Retsou et al. (Fri,) studied this question.