Response of Certain Biochemical Characteristics of Soybean Crop to Ascorbic Acid Spraying and Irrigation Interval

Abstract To find out how ascorbic acid spraying affects the biochemical characteristics of soybean crops under various irrigation interval impacts, as well as the extent to which the optimal ascorbic acid spray concentration may be recognized, a field experiment was conducted in the summer of 2023 at the College of Agriculture research station in the Al-Bu’itha area, Anbar Governorate. A randomized complete block design (RCBD) with three replications was used in the study’s split-plot layout. The experiment’s main plots had irrigation intervals of 4, 8, and 12 days, while ascorbic acid spray concentrations of 0, 1000, 2000, and 3000 mg L -1 were included in the sub-plots. The findings indicate that the concentration of ascorbic acid of 3000 mg L -1 surpassed the greatest mean in terms of chlorophyll content, carotene content, POD, and SOD achieving measurements of 32.1 mg g -1 fresh weight, 874.5 mg g -1 fresh weight, 56.7, and 0.811 respectively. In contrast same concentration of ascorbic acid recorded the lowest average of in terms of H 2 O 2 and MDA had 2.893 and 0.77 respectively. Whereas, irrigation interval the 4-day interval produced the best results in terms of chlorophyll content, and carotene content, were 39.37 mg g -1 fresh weight and 898.8 mg g -1 fresh weight respectively. While the 12-day interval recorded highest average of POD, SOD, H 2 O 2 and MDA had 74.011, 1.409,6.07 and 1.448 respectively.