Influence of dietary mulberry leaf on the nutritional composition of mulberry silkworm, Bombyx mori L. at different developmental stages

Abstract The distribution of mulberry leaf nutrients and trace elements into silkworm larvae over its development into pupa was investigated for potential novel food applications. Four stages of chronologically collected silkworm larvae, fed with mulberry leaves, were sampled. Proximate composition, vitamin A, β-carotene, mineral, fatty, and amino acid profiles were analyzed. The highest protein content was 63.68% in the fourth instar, whereas lipid was markedly highest at 31.85% in pupa. Magnesium and potassium were found to exceed the Thai recommended daily intake standard (RDI) in almost all stages, except for the potassium in the pupa. Vitamin A in the third to fifth instar was also found to be more than Thai RDI. The distinct highest zinc and fatty acid content were detected in the pupa. Monounsaturated fatty acids were detected at the highest levels in all stages. Sulfur-containing amino acids were abundant in the fifth instar. The fourth instar, the fifth instar, and the pupa are recommended as sources of protein, amino acids, fatty acids, and lipids in developing suitable food products.