The Contribution of Na+ or Cl− to Central NaCl−induced Cardiovascular Responses in Rodents

A high salt diet elevates plasma and cerebrospinal fluid NaCl concentrations in salt-sensitive hypertensive humans and animals. Acute or chronic intracerebroventricular (ICV) infusion of hypertonic NaCl elevates sympathetic nerve activity and blood pressure. However, it is unclear which ion, Na + or Cl − , contributes to this response. The purpose of the present study was to investigate the extent to which acute increases in cerebrospinal fluid Na + and Cl − increase ABP and HR in rodents. Male Sprague Dawley rats (n=6; body weight: 270-350 g) were anesthetized with isoflurane (2-3% in 100% O 2 ) and instrumented with a lateral ventricle cannula for ICV infusions. Additionally, arterial and venous catheters were implanted to record BP and administer drugs, respectively. At least 4 days after recovery, ABP and HR were measured in response to ICV infusion (10uL over 10 min) of the following solutions: artificial cerebrospinal fluid (aCSF); 0.3 M and 0.6 M NaCl; 0.15 M and 0.3 M Na 2 SO 4 , and 0.3 M and 0.6 M NH 4 Cl. Each animal received one ICV infusion of each solution every other day. Data are presented as mean ± SE. Baseline ABP and HR did not differ across each ICV infusion (ABP: from 98 ± 6 mmHg to 106 ± 6 mmHg; and HR: from 337 ± 17 bpm to 363 ± 12 bpm). As expected, ICV infusion of 0.3 M and 0.6 M NaCl produced concentration-dependent increases in ABP (Δpeak ABP 0.3 M NaCl: 17 ± 2 mmHg; 0.6 M NaCl: 26 ± 3 mmHg). While ICV infusions of Na 2 SO 4 at both concentrations also increased ABP, the magnitudes were reduced compared to NaCl (Δpeak ABP 0.15 M Na 2 SO 4 : 11 ± 2 mmHg; 0.3 M Na 2 SO 4 : 17 ± 3 mmHg). On the other hand, infusion of NH 4 Cl produced a modest increase in ABP (Δpeak ABP 0.3 M NH 4 Cl: 8 ± 2 mmHg and 0.6 M NH 4 Cl: 9 ± 3 mmHg). However, it did not differ from aCSF (Δpeak ABP aCSF: 6 ± 2 mmHg). HR responses induced by NaCl varied greatly (Δpeak HR 0.3 M NaCl: 15 ± 22 bpm; 0.6 M NaCl: 19 ± 17 bpm). In contrast, both concentrations of Na 2 SO 4 consistently increased HR (Δpeak HR 0.15 M Na 2 SO 4 : 64±10 bpm; 0.3 M Na 2 SO 4 : 60±10 bpm). Additionally, ICV infusions of NH 4 Cl also tended to enhance HR (Δpeak HR 0.3 M NH 4 Cl: 38 ± 13 bpm and 0.6 M NH 4 Cl: 41 ± 23 bpm). To end, ICV infusion of aCSF increased HR by 18 ± 3 bpm. These results suggest that the NaCl − induced pressor response is largely attributed to Na + . However, Na + alone cannot fully account for the NaCl − induced pressor response. NIH R01 HL163906, HL152680, HL145875. This is the full abstract presented at the American Physiology Summit 2024 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.