Antimicrobial Activity of Cream Containing Polyherbal Extract: Development, Formulation and Evaluation

Objective: To formulate and evaluate Antimicrobial herbal cream using Aloe vera gel, Turmeric, and dimethyl sulphoxide extracts of Neem (Azadirachta indica) and Tulsi extract (Ocimum tenuiflorum).Methods: Various herbal cream was prepared using the cream base, Aloe vera gel, turmeric, and dimethyl sulphoxide extracts of Neem and Tulsi containing aqueous phase and oil phase.The first three herbal creams C1, C2 and C3 were prepared with aloe vera gel, turmeric, and extracts of neem and Tulsi.Whereas the next three formulations C4, C5, and C6 contain marketed Tulsi drops instead of Tulsi extract.All the developed creams were evaluated according to various parameters viz.appearance, pH, clarity, viscosity, dye test, dilution test, spreadability, and antimicrobial activity against Gram-positive, Gram-negative bacteria and fungi.Results: Developed formulations C1, C2, C3, C4, C5, and C6 showed good appearance, adequate viscosity, pH, and were easily washable and no phase separation was observed.The formulations did not show redness, erythema, or irritation.All six formulations were found to be C1, C2, C3, C4, C5, and C6 and were stable at room temperature for one month.Developed polyherbal creams show good antibacterial activity against Gram-positive (Staphylococcus aureus), gram-negative (E.coli), and antifungal activity against Candida albicans and Aspergillus niger. Conclusion:All creams contain herbal ingredients that show good antimicrobial activity and formulations are stable at room temperature and would be further evaluated for preclinical studies.