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Abstract Background: In addition to primary injury, secondary injuries related to BBB disruption and immune-inflammatory response also play an important role in Intracerebral hemorrhage (ICH).And the Golgi apparatus play an important role in the state of ICH. Methods: ICH model and GM130-silencing ICH model were established in SD rats. The Garcia score was used to score the neurological defects of the rats. Blood- brain barrier (BBB) integrity were assessed by amount of extravasated Evans blue, and tight junction proteins. The expression of PD-L1 and GM130were detected through Western-blot and the subtype of microglia was showing with Immunofluorescence staining. Results: Compared with the ICH group, GM130-silencing ICH rats got a worsened neurological deficit and enlarged volume ofthe hematoma. Evan’s blue extravasation aggravated as well. The expression of GM130 in peri-hematoma tissue was further decreased, and the morphology and structure of the Golgi apparatus were further damaged. Meanwhile, the GM130 deficit resulted in decreased expression of PD-L1 and more polarization of microglia to the M1 subtype. Conclusion:We demonstrate that GM130 could influence the integrity of BBB and plays a role in neuroinflammation via regulation of PD-L1 after ICH. The manipulation of GM130 might be a promising therapeutical target in ICH.
Chen et al. (Wed,) studied this question.
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