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One of the global health challenges is the antibiotic-resistant pattern exhibited by Staphylococcus aureus and Pseudomonas aeruginosa. This study aimed to characterize S. aureus and P. aeruginosa from clinical specimens using molecular methods and to carry out plasmid profiles of antibiotic-resistant genes. Twenty isolates comprised of 10 S. aureus and 10 P. aeruginosa isolates from clinical specimens which exhibited multi-drug resistance were collected from the Department of Microbiology, Rivers State University. Polymerase chain reaction and DNA sequencing were employed to identify the isolates. Additionally, plasmid profiling was conducted to examine the presence of mecA, TEM, CTX-M and NDM-1 genes in the plasmid and the eno gene. Results showed that the 16S rRNA of the isolates showed a 99-100% similarity to the isolates of S. aureus with accession numbers (AF065394.1, NC007795.1, KV829593.1, NZJXHU01000160.1, KV841461.1, CP031670.1, CP031664.1, BX571856.1, AP017922.1 and CP003045.1) and P. aeruginosa (CP104590.1, NR026078.1, MN911415.1, CP007224.1, NZCP041945.1, NSPN01000008.1, NZNIZN01000087.1, KY086497.1, KJ482599.1, and KJ482590.1), respectively. Results of the plasmid profiling showed that 90% of the isolates possessed the blaTem gene, 40% of P. aeruginosa possessed New Delhi metallo-β-lactamase (NDM-1), 80% of both S. aureus and P. aeruginosa possessed the CTX-M gene while 40% of the S. aureus isolates possessed the mecA gene. Results also showed that 55% of all the isolates possessed the eno gene. The identification of plasmid-borne resistant genes emphasizes the need for continued surveillance and control measures to mitigate the spread of antibiotic resistance in healthcare settings.
Robinson et al. (Sat,) studied this question.