Investigating dOCT signal origins and bringing a new contrast to fluorescence imaging

Key Points

• The research aims to explore the origins of dOCT signals and enhance fluorescence imaging contrast.
• Investigated signal origins through dynamic optical coherence tomography and spinning disk confocal microscopy.
• Conducted correlative measurements to analyze findings.
• Proposed a framework for dynamic contrasting in imaging techniques.
• Dynamic contrasting improved the detection of organelle movement signatures.
• Organelle movements were accurately tracked in sub-second timeframes.