Abstract Background and Objectives To characterize and analyse 57 ABO subgroup samples from volunteer non‐remunerated blood donors in Ningxia, China, and elucidate the distribution profile of ABO subgroups in this region. Materials and Methods Routine ABO serological testing was performed on 568,286 blood donors from 2014 to 2024. Fifty‐seven samples exhibiting forward/reverse typing discrepancies underwent full‐length ABO gene sequencing using third‐generation single‐molecule sequencing technology. Results Among the 57 subgroup samples, phenotypes were distributed as follows: A2 ( n = 1), Aweak ( n = 10), Ax/Aweak ( n = 3), Bweak ( n = 10), Bel ( n = 4), A2B ( n = 5), AweakB ( n = 3), A1Bel ( n = 1), Ax/AweakB ( n = 3), A1Bweak ( n = 10), cisAB ( n = 3), B(A) ( n = 4). Third‐generation sequencing identified 11 A‐subgroup alleles, 10 B‐subgroup alleles and 3 cisAB/BA alleles. A novel allele was discovered, ABO*A.NEW , with a novel variant c.575T>C (NCBI accession number: PV290155). Conclusion The frequency of ABO subgroups in Ningxia blood donors was 1 per 10,000. A‐subgroups were predominantly ABO*AW.NEW (c.575T>C). B‐subgroups were primarily ABO*BW.12 . Complex glycosyltransferase alleles included ABO*cisAB.01 , ABO*BA.02 and ABO*BA.04 .
Liu et al. (Wed,) studied this question.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: