Digital Fluorescent pH Probes: Polymer-Based Design, Fluorescence Response, Mechanism, Functional Tuning and Application to Logic Operation in Live Cells

Key Points

• The research aims to develop polymer-based fluorescent pH probes for dynamic sensing and applications in live cells.
• Designed polymeric probes with thermoresponsive and proton-binding properties.
• Evaluated fluorescence response across different pH levels and temperatures.
• Tested probes in live cell environments to assess functionality.
• Achieved nearly complete fluorescence switching at targeted temperatures.
• Demonstrated functionality in live cells under varying pH conditions.
• Validated potential for use in complex cellular logic operations.