What question did this study set out to answer?

To explore the role of circRNA AIDA in regulating bovine myoblast development and its mechanistic interactions with miR-29a.

February 26, 2026Open Access

CircRNA AIDA Regulates Development of Bovine Myoblast via Binding miR-29a

Key Points

To explore the role of circRNA AIDA in regulating bovine myoblast development and its mechanistic interactions with miR-29a.
Identified circAIDA through bovine muscle sequencing data.
Investigated circAIDA's interaction with miR-29a and its downstream targets AKT3 and CLCN2.
Conducted in vitro assays to evaluate myoblast proliferation, apoptosis, and differentiation.
Executed in vivo experiments to assess muscle regeneration effects in mice.
CircAIDA promotes bovine myoblast proliferation and inhibits apoptosis and differentiation in vitro.
In vivo studies indicate circAIDA may hinder skeletal muscle regeneration in mice.
CircAIDA serves as a sponge for miR-29a, impacting its ability to repress targets like AKT3 and CLCN2.

Abstract

Circular RNAs (circRNAs) have emerged as critical regulators of skeletal muscle development, yet the functions of many muscle-derived circRNAs remain uncharacterized. In this study, we identified a novel circRNA, circAIDA, formed by exons 2 to 6 of the AIDA gene, based on bovine muscle sequencing data. Mechanistically, we demonstrate that circAIDA acts as a molecular sponge for miR-29a, thereby relieving the repression of its downstream targets, AKT3 and CLCN2. Functionally, circAIDA promotes bovine myoblast proliferation while inhibiting apoptosis and differentiation in vitro. Furthermore, experiments in vivo suggested circAIDA could attenuate regeneration of skeletal muscle in mice. In brief, we discovered a novel circAIDA/miR-29a interaction that regulates bovine myogenesis, providing new insights into the molecular networks controlling skeletal muscle development.

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