Histidines promote zinc over cadmium binding to the single type 4 metallothionein from Great Millet (Sorghum bicolor)

Key Points

• Zinc binding is favored over cadmium in the metallothionein of Sorghum bicolor, showcasing the importance of histidines.
• Specific interactions between histidines and zinc are critical for optimal metal binding in plant proteins.
• Observational analysis of metallothionein from Great Millet identifies key binding preferences for metal ions.
• Understanding these preferences supports insights into plant metal homeostasis and protection against toxicity.