Development and validation of a digital droplet PCR method for the detection of Lactococcus garvieae/petauri in water, head kidney and cloacal swabs of infected rainbow trout (Oncorhynchus mykiss)

Key Points

• Detection of lactococcus garvieae is confirmed in water and trout samples, indicating environmental and health risks.
• The digital droplet PCR method shows high sensitivity, achieving quantification limits as low as 1 cell per mL in samples.
• Validation involves a comprehensive analysis of water, head kidney, and cloacal swabs from infected rainbow trout.
• Effective monitoring allows for early detection of potential outbreaks in aquaculture, helping maintain fish health.