A one-step, extraction-free RPA-CRISPR/EsCas13d platform for rapid, sensitive and visual detection of PCV2

Key Points

• Rapid detection of PCV2 achieved using a one-step RPA-CRISPR/EsCas13d platform—offering high sensitivity.
• Sensitivity of the method allows for effective detection, crucial for timely responses in veterinary diagnostics.
• Implementation of the extraction-free approach streamlines the process, reducing time and resource requirements.
• The technique may enhance diagnostics in veterinary medicine, particularly for PCV2-related diseases.