Fluorometric determination of lasR gene transcription in Pseudomonas aeruginosa via duplex-specific nuclease-assisted recycling and flap endonuclease 1–driven DNA walker cascade amplification

Key Points

• Transcription levels of the lasR gene are significantly detectable using a fluorometric approach, enhancing sensitivity.
• The method incorporates duplex-specific nuclease and flap endonuclease 1 for DNA amplification, ensuring accuracy.
• The fluorescent signal provides a reliable measure of lasR gene activity, crucial for understanding bacterial behavior.
• Improved detection capabilities may enhance strategies to combat Pseudomonas aeruginosa infections.